Table 2.
In vitro screening of heterologously expressed UGTs that showed the most significant changes in expression level between sur2 knock-out mutant and wild-type towards a set of six commercially available indole and phenylpropanoid compounds.
Name | Group | S1 | S2 | S3 | S4 | S5 | S6 |
UGT73B2* | D | +++ | (+) | (+) | ++ | + | (+) |
UGT73B3 | D | (+) | − | − | +++ | (+) | (+) |
UGT73B5 | D | (+) | − | − | − | − | (+) |
UGT72B1 | E | (+) | − | (+) | − | +++ | − |
UGT72E2 | E | − | − | − | − | (+) | − |
UGT71C3* | E | +++ | − | − | + | ++ | (+) |
UGT74F1 | L | +++ | (+) | (+) | ++ | (+) | − |
UGT75B1 | L | +++ | +++ | − | (+) | − | +++ |
UGT84A2 | L | +++ | (+) | − | (+) | ++ | +++ |
UGT84A3 | L | ++ | (+) | (+) | − | − | +++ |
S1: indole-3-carboxylic acid; S2: indole-3-acetic acid; S3: indole-3-carboxaldehyde; S4: coniferyl alcohol; S5: sinapoyl-alcohol; S6: sinapic acid.
* UGTs that were not identified based on their direct up-regulation, but were selected because of their homology to other up-regulated UGTs (Table 3). Based on the intensity of the signal corresponding to the formation of a glucosylated product, a relative scale was established: +++: high conversion; ++: medium conversion; +: low conversion; (+): minute conversion; -: no conversion.