Table 2.
Metabolite number | Metabolite name | C24-crosses | Col-crosses |
VIP | VIP | ||
3 | Fumaric acid | 2.2143 | 1.4084 |
7 | Succinic acid | 2.0124 | not selected |
8 | Fructose | 1.6657 | 1.7774 |
9 | Galactose | 1.6557 | 1.9322 |
10 | Glucose | 1.6072 | 1.2923 |
13 | Raffinose | 2.1335 | 2.7135 |
14 | Sucrose | 1.0619 | 1.2245 |
18 | Galactinol | 1.5055 | 2.1000 |
20 | Maltitol | Not selected | 1.1885 |
21 | Glycine | 1.0737 | Not selected |
22 | Proline [+CO2] | 1.1567 | Not selected |
23 | Proline | 1.5450 | 1.3678 |
30 | Dehydroascorbic acid dimer | 1.1416 | 1.3948 |
36 | Hexadecanoic acid | 0.9456 | Not selected |
38 | Itaconic acid | 0.9644 | Not selected |
39 | Ethanolamine | 1.0949 | Not selected |
48 | NA | 1.6438 | 1.6560 |
50 | NA | 0.9593 | 1.2818 |
51 | NA | 0.9857 | 1.2442 |
52 | NA | 1.0154 | Not selected |
59 | NA | 1.0534 | 1.5046 |
Data from both non-acclimated and acclimated plants were combined. Freezing tolerance data from electrolyte leakage measurements were taken from Korn et al. (2008) (compare Supplemental Table 2). The calculations were performed separately for Col-0- and C24-crosses and included the respective parental accessions, since the LT50 values for these groups were measured separately, leading to a small systematic shift in all LT50 values (C24-crosses: N = 20 (including C24×Col and Col×C24); Col-crosses: N = 14). Metabolite numbers refer to the numbers in Supplemental Table 1. VIP, variable importance in the projection. Higher VIP scores indicate greater importance of the selected metabolite in the prediction model. All 59 metabolites were included in the analysis for the C24-crosses, while for the Col-crosses, tryptophan was excluded because of more than 10% missing values.