Table 1.
Drought responsive cDNA-based primer sequences and restriction enzyme combinations used for restriction site polymorphism mapping in a recombinant inbred population developed from a cross between homozygous cowpea genotypes IT93K503-1 and CB46
| cDNAa | Forward primerb | Reverse primerb | Restriction enzyme combination | Polymorphic fragments amplified | References |
|---|---|---|---|---|---|
| ACCc | CGAYGGSTGGAAGGCBTAYG | YCTSRTYCACATCGYSTACA | EcoR1 + Mse1 | 3 | – |
| CPRD8c | CAAYGTTCCRCARCCYCAYGC | TWCTRTSRYGGRAAYCGTACTTG | EcoR1 + HpaII | 2 | – |
| CPRD12c | GGTATWMGKGTGAACKGTGTKTC | YWTKTCKYGWCGAAGACGGTGTAS | EcoR1 + Mse1 | 3 | – |
| CPRD14c | GACAAGTAGTGTGTGTTACC | ACAGTATCCTTGATGCTCAC | EcoR1 + Msp1 | 3 | – |
| CPRD22c | CAAGTTACCAGAAGCAGTAC | CCACATTTACACGACAAGAC | EcoR1 + Mse1 | 4 | – |
| CPRD46c | GGSTAYKTYCCMAAYMGRCCA | GRAGYAGDCCKCTYACKMRGCT | EcoR1 + Msp1 | 2 | – |
| P5Cc | ATTTGARRTBATWYTGGTRW | AAATWYTRYTDRMWATDGCY | EcoR1 + Msp1 | 1 | – |
| VuAPXc | AGGYHTCATCGCYGARAAGA | TTTTGTCHGAYCCTGTVTTC | EcoR1 + Mse1 | 1 | – |
| VuC | CGYTTYGCYGTBGARGA | CCCAVACYTTDGCYTCRTA | EcoR1 + HpaII | 0 | Diop et al. (2004) |
| VuNCED1 | ATTGAATTCATGCCTTCAGCTTCAAAC | ATTGGATCCCAAAAGCTACACGCTGGT | EcoR1 + Mse1 | 3 | Iuchi et al. (2000) |
| VuPAT1 | GAYTAYTTYGAYRTNATCG | GGRKSNCCNAARATHTTYCC | EcoR1 + Msp1 | 3 | Matos et al. (2001) |
| VuPI-PLC | ACSGGSCAYAAYWSSTAYYT | GGCCASARRTCSARYTCDATSAC | EcoR1 + Mse1 | 0 | El-Maarouf et al. (2001) |
| VuPLD1 | CAYCAYGAYGAYTTYCAYCARCC | TAYTCRTCRTCNACDATCATCAT | EcoR1 + Msp1 | 2 | El-Maarouf et al. (1999) |
aSee text for full names of cDNA clones
bB = C/G, D = A/G, H = A/C/T, K = G/T, M = A/C, N = A/C/G/T, R = A/G, S = C/G, W = A/T, and Y = C/T
cNew primers were designed, see section “Materials and methods”