Figure 2.
miR-183 regulates the splicing factor SC35. a QRT-PCR for miR-183 in CHO cells transfected with pre-miR-183 or anti-miR-183 (Ambion) and GFP-transfected controls. Bars: SD; t test p < 10−5 for both concentrations of pre-miR-183 compared to GFP control. b Scan and quantification of immunoblot for SC35 in cells in a with α-tubulin used for normalization. c Suggested interplay of alternative splicing and miRs (exemplified by miR-134, miR-183, and their target, the splicing factor SC35) contributes to the complexity of stress responses in the brain, including regulation of the alternative splicing choice between the major “synaptic” AChE transcript AChE-S and the normally minor stress-induced variant AChE-R