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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Clin Cancer Res. 2010 Jan 12;16(2):600–609. doi: 10.1158/1078-0432.CCR-09-1979

Figure 1. SLPI increases cell proliferation.

Figure 1

A. Forced expression of HA-tagged SLPI stimulates growth. Inset shows HA tag expression. P-values represent the statistical significance for the entire curve. B. CR012 recognizes SLPI. CR012 is credentialed by its recognition SLPI by immunoprecipitation/immunoblot in OVCAR3 lysates (lanes: 1-SLPI antisense-treated cells, 2-scramble-treated, 3-transfection medium control, 4-no exposure), and it illuminates membrane-bound SLPI (B; blue: untreated; green: IgG2 control; red: CR012, 2ug/mL). C, D. CR012 is a neutralizing anti-SLPI antibody. CR012 blocks SLPI inhibition of elastase activity in vitro (C) and reduces proliferation in the presence or absence of added SLPI (D). IgG2 isotype control is used to show specificity. Representative data of at least three independent experiments are shown.