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. 2009 Nov 12;10(11):4963–4976. doi: 10.3390/ijms10114963

Figure 1.

Figure 1.

Formation of a 3D-like proliferation pattern of NHDFs mediated by pressed silk. Upper panels (a, b): Confluent cultures of NHDFs were trypsinized and seeded into flasks with pressed silk under the conditions of heat treatment at 43 ºC for 10 min and cultured for one week (a) or two weeks (b). Lower panels (c, d): Phase-contrast photomicrographs of cells that had been heat-treated at 43 ºC for 10 min after two weeks (c) and three weeks (d) of culture with pressed silk (original magnification, ×40).