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. 2009 Nov 12;10(11):4963–4976. doi: 10.3390/ijms10114963

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Stimulation of DNA synthesis by heat treatment. NHDF cells were plated on sterile glass coverslips coated with poly-D-lysine and were heat-treated or not heat-treated and then pulsed for 60 min with 10 μM BrdU. Immunostaining was performed using an anti-BrdU kit and was observed using a fluorescent microscope (200×).