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Morphology of intact cells and nuclear morphology of apoptotic cells. Chamber slide culture cells that had been incubated for 3 days following heat treatment at 45 ºC for 20 min displayed apoptotic morphologies. The morphology of these cells was determined by TUNEL staining and fluorescence microscopic observation and was shown in Fluorescein (a) and Fluorescein/Phase contrast image (b) (×200).
