Figure 5.

Survival curve of heat-treated NHDFs. Cells were trypsinized and plated in triplicated in 60-mm-diameter Corning dishes. After allowing attachment of the cells for 4 h or after allowing culture of cells for 24 h (•), the cells were heat-treated at 40, 43, 45 or 47 ºC for 10 min in the presence (♦) or absence (⋄) of the Hsp inhibitor KNK437 at 3.6 μM. The heat-treated cells were incubated for 10 days until colony staining. Each value is the mean ± S.E.M. for five independent experiments.