Figure 2. Effect of SOCE inhibitors on thapsigargin (TG) induced entry and agonist-induced Ca²⁺ oscillations.

A) Shown is a trace of 30-35 hepatocytes, representative of 3 experiments, under control conditions (filled squares) or pre-treated with either 30 μM 2APB (open circles) or 1 μM Gd³⁺ (open triangles) before adding thapsigargin in the absence of Ca²⁺ followed by addition of 1.8 mM Ca²⁺. Treatment with 2APB or Gd³⁺ completely blocks the Ca²⁺ entry. B-C) The frequency of vasopressin-induced oscillations is diminished by 2APB or Gd³⁺. The data is taken from 3 independent experiments and is reported as the mean ± SEM, where N=54 and 50 cells for 2APB or Gd³⁺, respectively. One-Way ANOVA revealed which time points had a significant change in the number of Ca²⁺ spikes, denoted by “*” (p < 0.05).

D) ATP-induced oscillations in Ca²⁺ free media or 1 μM Gd³⁺ follow trends similar to that observed when using vasopressin. The data, taken from 3 independent experiments, is reported as the mean ± SEM where N=98, 82, and 60 for 5 μM ATP, 1 μM Gd³⁺, or nominally-free Ca²⁺, respectively. One-Way Anova revealed a significant change in the number of Ca²⁺ spikes for each time point, denoted by “*” (p < 0.0001).