Figure 1.

Transcripts of Notch signaling components were increased in fibrotic peritoneum induced by PDF. A: Normal rats (A1 to A6) or rats that received daily intraperitoneal injections of 4.25% Dianeal (100 ml/kg) for 14 days (B1 to B6) or 28 days (C1 to C6) were sacrificed, mRNA was collected, and RT-PCR was performed to detect mRNA level of Notch-1, Jagged-1, and HES-1. GAPDH was used to verify equivalent loading. Each panel represents an independent experiment with two animals per group. B: Graphic representation of relative abundance of Notch-1, Jagged-1, and HES-1 normalized to GAPDH. Data are given as mean ± SD (n = 6). *P < 0.05 versus normal rats.