Figure 14.

Myosin, actin, and desmin around intercalated disk in I-mode and IV-mode. Myosin, actin (HHF35), and desmin are individually immunohistochemically stained with paraffin section (nonperfused fixed hearts). They are regularly stained in the myocyte body; however, around ICDs, which we suppose to exist in the zones indicated by arrows, myosin, actin, and desmin show characteristic features as follows. In I-mode [control, 2-day after AVF (2-day), 4-day after AVF (4-day) and 4-day after overload removal of 4-day overloaded animal (4-day + 4-day)], the zones are narrow and simple. They are interposed by two myosin stained lines and two desmin stained lines. They are vaguely stained by actin. In IV-mode [1-day after AVF (1-day), 3-day after AVF (3-day), 1-day after overload-removal of 4-day overloaded animal (4-day + 1-day)], the zones are wide and irregular. Nearly two sarcomere-wide zones appear between myosin stained lines. Actin is irregular and vague and desmin is irregular in these zones.