Figure 4.

Effects of sFas gene delivery on expression of Fas, Fas ligand and apoptosis-related mediators in acute doxorubicin cardiotoxicity. A: Western blots for Fas and Fas ligand. B: Double immunofluorescence for TUNEL (green) and myoglobin (red) to separately evaluate TUNEL positivity for cardiomyocytes and nonmyocytes. Arrows point to TUNEL-positive cells. The left panel shows a TUNEL-positive cardiomyocyte while the right panel does a TUNEL-positive nonmyocyte. Scale bars = 10 μm. C: Western blots for caspase-8 and -3 and their uncleaved (pro-) forms. Densitometries of their cleaved (activated) forms are not shown because they were undetected. D: Western blots for Bcl-2 and Bax and their densitometric analyses. *P < 0.05 versus the sham group; ^#P < 0.05 versus the doxorubicin-treated group with LacZ gene transfer.