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. 2010 Feb;176(2):721–732. doi: 10.2353/ajpath.2010.090454

Figure 3.

Figure 3

The effect of DKK1 on fibroblast proliferation and actin bundle formation of fibroblasts. A, B: The fibroblasts from a healthy individual (control) and an individual with PDP (PDP) were incubated with or without recombinant human DKK1 protein and the DNA contents of fibroblasts were evaluated with 7-AAD using flow cytometry. Representatives of FACS plots of fibroblasts from a healthy individual (control) and an individual with PDP (PDP) are shown (A). The percentages of fibroblasts in S-G2 phase in triplicated wells are expressed as the mean ± SD (n = 3). The student’s t-test was performed between the indicated groups and *P < 0.05. C: The actin bundle formation of cultured fibroblasts from a healthy individual (control) and an individual with PDP (PDP) were examined by staining with alexa 488-labeled phalloidin antibody 5 days after the fourth passage. Upper panels, low magnification (×10); lower panels, high magnification (×40).