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. 2010 Feb;176(2):827–838. doi: 10.2353/ajpath.2010.090006

Figure 4.

Figure 4

TN-C promotes luminal filling and upregulates c-met. A: Confocal immunofluorescence staining with laminin-V (green; upper) and DAPI (nuclei, blue) revealed changes in lumen structure (white dotted lines) in acini cultured with or without TN-C. C-met staining intensity is increased in the presence of TN-C (lower right) when compared with control (lower left). Scale bars, upper panels, 50 μm; lower panels, 25 μm. B: Western blot analysis confirmed that c-met levels are increased in the presence of TN-C, whereas secreted HGF levels remain unchanged. GAPDH is the loading control. C: Densitometric analyses of c-met levels relative to GAPDH reveal a significant 1.8-fold increase in the presence of TN-C. D: Tissue sections from the normal human mammary gland (top) and grade 2 IDCs (middle and bottom) stained with H&E (left) or c-met and TN-C (red and blue, respectively; right). Scale bar, 50 μm.