Figure 2.

Inhibition of CD36 by PGE₂ is mediated by the EP2 receptor signaling pathway. A: Macrophages isolated from normal women were treated with vehicle, PGE₂ (1 μM), or selective agonists for EP1 (ONO-D1-004, 10 μM), EP2 (ONO-AE1-259-01, 10 μM), EP3 (ONO-AE-248, 10 μM), or EP4 (ONO-AE1-329, 10 μM) for 12 hr. CD36 mRNA expression was detected by real-time quantitative RT-PCR. B: Macrophages isolated from normal women were treated with PGE₂ (1 or 10 μM) or PGE₂ plus inhibitors as indicated for 12 hr, and CD36 mRNA expression was determined by real-time quantitative RT-PCR. C: Macrophages isolated from normal women were treated with vehicle (Con), PGE₂, or PGE₂+AH6809 (10 μM) for 96 hr, and CD36 expression was assessed by staining with anti-CD36 antibody or isotype-matched FITC-IgA (isotype control) followed by quantification via flow cytometry. Representative flow cytometric data are shown in the left panel. The right panel shows the mean and SD of four independent experiments using different batches of peritoneal macrophages. D: Representative confocal images show CD36 staining in macrophages isolated from normal women treated with vehicle (Con), PGE₂, or PGE₂+AH6809 as described in (C). This experiment was done four times using different batches of cells. *P < 0.05, **P < 0.01, ***P < 0.001.