Table 1.
Gene Symbol | NCBI ref # | Sense | Antisense | Amplicon length (bp) |
---|---|---|---|---|
Human | ||||
CD36 | NM_000072 | 5′-AGATGCAGCCTCATTTCCAC-3′ | 5′-CGTCGGATTCAAATACAGCA-3′ | 101 |
SCARB1 (SR-BI) | NM_001082959 | 5′-AGATCCTGAAGGGCGAGAAG-3′ | 5′-CGGTGTCGTTGTTGTTGAAG-3′ | 102 |
SCARB2 (SR-BII) | NM_005506 | 5′-CCAAATCAGGAAGACCATGAG-3′ | 5′-TCCCGTTTCAACAAAGTCATC-3′ | 126 |
MSR1 (SR-AI) | NM_138715 | 5′-CTGGTCCAATATGGCTGAATG-3′ | 5′-CAGAATGTGAACAGGCTCTTG-3′ | 102 |
MARCO (SR-AII) | NM_006770 | 5′-GAGTGGAAACACATTAAGACCA-3′ | 5′-ATGACTGTCCAGAGGTGAAG-3′ | 133 |
SCARA3 (SR-AIII) | NM_182826 | 5′-TTTCCTGGAAGTCGAGGAC-3′ | 5′-GCCATATTGGACCAGTACTTA-3′ | 115 |
RN1BS1 (IBS) | NR_003286 | 5′-GTGTGCCTACCCTACG-3′ | 5′-TGACCCGCACTTACTC-3′ | 114 |
OAS1 | NM_016816 | 5′-TGGTTGTCTTCCTCAGTCCTC-3′ | 5′-CTCTCTTTGACAGGCTTCACG-3′ | 104 |
PTGER1 (EP1)* | NM_000955 | 5′-CTGCCCATCTTCTCCATGAC-3′ | 5′-CCGGGTACTGCAGCTCATAG-3′ | 442 |
PTGER2 (EP2)* | NM_000956 | 5′-TGCTGCTTCTCATTGTCTCG-3′ | 5′-CGACAACAGAGGACTGAACG-3′ | 388 |
PTGER3 (EP3)* | NM_000957 | 5′-ATGCTGCTCACTGGTTTCG-3′ | 5′-TCTTCATGTGGCTCGCATAC-3′ | 340 |
PTGER4 (EP4)* | NM_000958 | 5′-TGTGAACCCCATCCTAGACC-3′ | 5′-CCAAGGCCATTTTCACTGAG-3′ | 273 |
Mouse | ||||
Cd36 | NM_007643 | 5′-CTTGAAGAAGGAACCACTGC-3′ | 5′-GTTCTTTGCCACGTCATCTG-3′ | 108 |
Scarb1 (SR-BI) | NM_016741 | 5′-TTTATGAACCGCACAGTTGG-3′ | 5′-TTTATGGGAAGCATGTCTGG-3′ | 83 |
Scarb2 (SR-BII) | NM_007644 | 5′-TTATCGAAGCCATGCTGAAAG-3′ | 5′-AAATATGGACGAGGGACAAGA-3′ | 108 |
Msr1 (SR-AI) | NM_031195 | 5′-GGAAGAAGAAGAGGGTCTGTCA-3′ | 5′-CTGGCTCAAGCTGTTGTCAT-3′ | 85 |
Gapdh | NM_008084 | 5′-GGAGATTGTTGCCATCAACG-3′ | 5′-GAATTTGCCGTGAGTGGAGT-3′ | 85 |
All primers listed were used for both conventional and real time PCR except those labeled with asterisks.
Primers for conventional PCR.