Figure 1.

Active forms of GSK3β detected in brain endothelium in HIV encephalitis (HIVE). Five-μm-thick frozen sections of control human brains (A–D) or HIVE (E–P) were cut. The sections were triple immunostained with Ulex europeus agglutinin 1 (endothelial marker, red, A, E, I, M) and anti–p-GSK3 antibodies (green, B, F, J, N) or DAPI (blue, C, G, K, O). Arrows depict locations of microvessels in panels A–P. Microvessels in control brain tissue (A) showed minimal staining for p-GSK3β (B, green, arrow). In HIVE, the brain endothelium (E, I, M) featured a high level of p-GSK3β (green, arrows, F, J, N). Original magnification: ×200 (A–P).