Table 2.
Immunofluorescence Primary Antibodies
| Antibody (Clone) | Source | ID | Type | Dilution |
|---|---|---|---|---|
| Immunoproteasome | ||||
| LMP2/β1i | Abcam Cambridge, MA | ab3328 | Rabbit pAb | 1:1000 |
| PA28α | Biomol | PW8185 | Rabbit pAb | 1:1000 |
| Neurons | ||||
| NeuN (A60) | Millipore Billerica, MA | MAB377 | Mouse mAb | 1:100 |
| Neurofilament (SPM204) | Abcam | ab17832 | Mouse mAb | 1:50 |
| Synaptophysin (SVP-38) | Sigma Aldrich | S5768 | Mouse mAb | 1:200 |
| Astrocytes | ||||
| GFAP (GA5) | Millipore | MAB360 | Mouse mAb | 1:400 |
| Oligodendrocytes | ||||
| OMG (4A9) | Lifespan Seattle, WA | LS-C27282 | Mouse mAb | 1:300 |
| Macrophage/microglia | ||||
| CD68 (KP1) | Dako | M0814 | Mouse mAb | 1:100 |
mAb, monoclonal antibody; pAb, polyclonal antibody.