Figure 3.

CTL activity in splenocytes removed from BALB/c mice 2 weeks after immunization of 7-day-old mice with protein-alum-CpG (•), protein-alum (■), protein-CpG (□), or a DNA vaccine (○). All mice were given in vivo restimulation (1 μg HBsAg without adjuvant) 3 days before removal of the spleen. Each point represents the mean of values obtained from CTL-positive mice that were each assayed in triplicate. Fraction of CTL-positive mice is indicated for each line. E/Ts are indicated on the horizontal axis. The vertical axis shows HBsAg-specific lysis as a percentage of the total possible lysis.