Figure 7.
Effects of anti-human IL-6 antibody (hIL6Ab) and anti-mouse IL-6 antibody (mIL6Ab) on Rankl mRNA expression induced by BHY-CM (A) and Ca9-CM (B) in ST2 cells. ST2 cells were cultured for 2 days in the presence or absence of each CM, and the Rankl mRNA expression in ST2 cells was determined by using mouse specific primers. *P < 0.01, **P < 0.05. C: Effect of anti-human PTHrP antibody on mouse Il-6 mRNA expression induced by CM-isolated from OSCC cell lines in ST2 cells. ST2 cells were cultured for 2 days in the presence or absence of CM isolated from each OSCC cell line, and the effect of anti-PTHrP antibody on Il-6 expression was determined. In each experiment, the cells were treated with 10 μg/ml each antibody. Black bars, values treated with each CM only; gray bars, values treated with each CM and anti-PTHrP antibody; white bars, values treated with each CM and normal mouse IgG1 (10 μg/ml). *P < 0.05, significantly different from each CM induced Il-6 mRNA expression.