Table 1.

Genes Significantly Regulated in Opposite Ways by FAC and Dfo in Two Independent Lines of SV40 Hepatocytes

Gene	Dfo/FAC ratio
Mdm-2	3.3
Envoplakin-1	2.7
p21	2.4
CyclinG1	2.3
Ero-1 like	1.5
TfR-1	2.5

The relative mRNA expression in Dfo versus FAC treated cells is shown on the right; mRNA levels of TfR1 are shown as a control for iron status. Experimental design. Step I: gene expression evaluation in iron supplemented, control, and iron depleted cells in two cell lines. Step II: Identification of genes modulated by iron depletion/ supplementation versus control. Step III: Identification of genes modulated in opposite ways by iron depletion versus iron supplementation. Step IV: Identification of genes modulated in opposite ways in both of the two cell lines evaluated.