Fig. 1.

DIM decreases the levels of the class I HDACs. (A) HT-29 cells were treated with various doses of DIM for 24 hours. Western blotting was done using the indicated antibodies. (B) SW620 cells were treated with various doses of DIM for 24 hours. Western blotting was done using the indicated antibodies. (C) Nude mice bearing HT-29 xenografts were treated with DIM at oral doses of 100, 200, 300 mg/kg/day for two days. Tumor samples were collected 24h after the second dose and analyzed by western blotting. Representative results from three independent blots were shown.