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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2009 Oct;8(10):2882–2893. doi: 10.1158/1535-7163.MCT-09-0293

Figure 3. SGI-1776 treatment causes cell cycle arrest and apoptosis in prostate cancer cells.

Figure 3

(A) Western blot analysis of phospho-p21Thr145 and total p21 was performed on C4-2B and 22Rv1 cells after a 5 hour treatment with SGI-1776. β-actin was used as a lane loading control. (B) Flow cytometry analysis of C4-2B and 22Rv1 treated with SGI-1776 for 24 hours was performed. Results from three different experiments were averaged and displayed in this figure. (C) Western blot analysis probing for phospho-BadSer112 and total Bad was performed on C4-2B and 22Rv1 cells treated with increasing doses of SGI-776 for 5 hours. β-actin was used as a lane loading control. (D) Caspase-3 activity was measured using a fluorometric protease assay after treatment with increasing doses of SGI-1776 for 24 hours. The assay was run in duplicate and the results were normalized to the untreated samples.