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Exposure of human monocytes to TLR ligands, but not to IL-6, increases surface expression of the procoagulant TF. Whole blood was obtained from HIV-uninfected subjects and was exposed to LPS (50 ng/mL), flagellin (10 μg/mL), or IL-6 (30 μg/mL) for 3 hours. Surface expression of TF was measured on CD14+ monocytes by flow cytometry. (A) Gating strategy. (B) Representative histograms. (C) Summary data.
