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Loss of p53 delays Puma up-regulation in IL-3–deprived FDM cell lines. (A) Cells of the indicated genotypes were deprived of IL-3 over a 48-hour time course. Lysates were prepared, and proteins were resolved on SDS-PAGE. Western blots were probed with antibodies to Puma, Noxa, and β-actin (loading control).
