Table 2.

UFM diagnosis of coding strand in CDSs with size (Bp) varying between 150 and 400 bp in the species (Sp) H. sapiens (Hs), D. melanogaster (Dm) and A. thaliana (At). The sample size (N) was 1000 throughout the experiment.

Sp	Bp	N	ΔF + ≥11	Bp + = Bp−2	ΔF −≥ 13	F + 1≥14	F−1≥15	Fr−16	Fr−2	Fr−3	Fr+1 ≥ Fr−1	F+1 < F−1	Fr−1	Fr−2	Fr−3
Hs	400	1000	1000	202	202	1000	178	122	44	12	981	197	6	12	1
Dm		1000	1000	214	214	998	204	161	37	6	979	20	10	7	3
At		1000	1000	30	30	999	29	21	8	0	992	7	5	2	0
Hs	350	1000	1000	254	254	999	223	151	56	16	971	29	8	20	1
Dm		1000	1000	264	264	999	257	197	50	10	973	26	14	9	3
At		1000	1000	50	50	998	49	33	15	0	993	5	4	1	0
Hs	300	1000	1000	314	314	999	271	179	72	20	957	43	14	26	1
Dm		1000	1000	342	342	999	327	241	71	15	961	38	17	16	5
At		1000	1000	97	97	998	96	56	33	7	962	19	7	10	2
Hs	250	1000	1000	388	388	998	326	211	92	23	943	57	15	38	4
Dm		1000	1000	428	428	997	406	301	90	15	946	54	25	24	5
At		1000	1000	152	152	996	146	79	59	8	981	36	14	19	3
Hs	200	1000	1000	538	538	996	460	273	152	35	900	100	32	60	8
Dm		1000	1000	566	566	992	519	354	133	32	926	72	32	36	4
At		1000	1000	249	249	993	242	124	98	20	929	69	28	35	6
Hs	150	1000	1000	699	699	994	590	333	198	59	842	158	53	87	18
Dm		1000	1000	723	723	986	651	398	204	49	865	135	44	82	9
At		1000	1000	430	430	989	412	186	183	43	868	129	40	70	19

¹“ΔF+” is for PBI considering the “+” strand.

²“Bp+ = Bp−” is to indicate that ORF size is the same for “+” and “−” strands.

³“ΔF−” is for PBI considering the “−” strand.

⁴“F+1” is for f₁ considering the “+” strand.

⁵“F−1” is for f₁ considering the “−” strand.

⁶“Fr−” is for the real frame of the CDS, i.e. Fr−1 for frame −1, Fr −2 for frame −2 and Fr −3 for frame −3, respectively,

⁷The gray area is for error rate of coding frame diagnosis ≤5%.