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. 1998 Dec 22;95(26):15559–15564. doi: 10.1073/pnas.95.26.15559

Figure 3.

Figure 3

Privileged site coding joint formation in SCID mice does not depend on residual DNA-PKcs functions. Thymocyte DNA samples were subjected to semiquantitative PCR analysis for TCR Dδ2-Jδ1 coding joints. Titrations with wild-type DNA (100, 10, 1, 0.1 ng; lanes 1–4, respectively) were performed to estimate the abundance of coding joint formation in SCID (lane 5) and three SLIP (lanes 6–8) DNA samples (all at 100 ng). The expected size is 199 bp. A negative PCR control (all reagents without DNA) is shown in lane 9. Relevant sizes of the DNA marker are indicated adjacent to lane M.