Skip to main content
. 2010 Jan 19;8(1):e1000291. doi: 10.1371/journal.pbio.1000291

Figure 4. HSF1A suppresses aggregation and cytotoxicity in a cell culture model of Huntington disease.

Figure 4

(A) HD-Q74 PC12 cells were incubated with increasing concentrations of HSF1A for 15 h or heat shocked for 2 h at 42°C, followed by a 15-h recovery and Hsp70 levels analyzed by immunoblotting, with SOD1 as a loading control. (B) PC12 cells were pretreated with either DMSO or 10 µM HSF1A for 15 h and doxycycline added to 1 µg/ml, with further incubation for 48 h. Equal amounts of the soluble and insoluble fractions were analyzed for the presence of httQ74-GFP by immunoblotting with anti-GFP antibody. (C) Fluorescence pattern for httQ74-GFP analyzed microscopically in cells pretreated with DMSO or HSF1A prior to induction of httQ74-GFP expression. (D) Quantification of (C) by counting the number of cells containing aggregates expressed as a percentage of the total number of cells counted. For each treatment, approximately 800 HD-Q74 PC12 cells were evaluated. **p<0.01; ***p<0.001. (E) PC12 cells were pretreated with 4 µM HSF1A for 15 h, before the addition of doxycycline (Dox) to 1 µg/ml followed by a 5-d incubation. Cell viability was assayed by the XTT viability assay. ***p<0.001.