Figure 5.

Caspase-8 enhances neuroblastoma metastasis under nonapoptotic conditions in a Rab5-dependent manner. Control shRNA (black bars) or Rab5-shRNA–treated cells (open bars) either lacking (−) or expressing caspase 8/C360A (C8i) were seeded into the upper chorioallantoic membrane of 10-d-old chick embryos, and tumors were permitted to develop for 8 d. (A) Total cumulative incidence of detected human Alu sequences, (expresses as percentage) from genomic DNA isolated from lungs of the tumor-seeded chick cohorts was measured by polymerase chain reaction. Statistical comparisons between cohorts representing three experiments were done via unpaired t test. (#p = 0.30; **p = 0.09; *p = 0.07). (B) Tumors were resected from the chorioallantoic membrane, and the wet weight was determined (mean ± SE).