Figure 6.

RHO-1 and CDC-42 pathway components are required to recruit GFP::NMY-2 to the cortex in distinct phases. Optical sections through the cell cortex of embryos expressing GFP::NMY-2 in the early embryo. In the wild type, GFP::NMY-2 localizes to the cortex in distinct morphological distributions in phases I and II. The robust myosin foci observed in phase I depend upon ECT-2, RHO-1, and LET-502 [represented here by let-502(fRNAi)]. The robust, diffuse, and polar distribution of smaller myosin puncta in phase II depends upon CGEF-1, CDC-42, and MRCK-1 [represented here by mrck-1(ok586)]. Disruption of either pathway does not abolish the recruitment by the other, and disruption of both pathways disrupts both recruitment patterns. Shown here are embryos from wild-type, mrck-1(ok586), let-502(fRNAi), and ect-2(fRNAi); cdc-42(fRNAi) mothers. Embryos from each of the six single disruptions are presented in Supplemental Figure 3. Bar, 10 μm.