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. 2010 Jan 19;5(1):e8760. doi: 10.1371/journal.pone.0008760

Figure 2. Differential expression of genes in skeletal muscle of TWEAK-Tg mice.

Figure 2

Gastrocnemius muscle of 6 months old TWEAK-Tg mice and littermate control mice were used for total RNA isolation and QRT-PCR analysis. The relative expression values from the QRT-PCR analysis were plotted for each gene are mean ± SD (n = 3). The numbers above the bar represents the fold changes in TWEAK-Tg against littermate control mice, and ‘*’ represents the statistical significance (p-value ≤0.01). A) The levels of Nfkbia, Nfkb2, and Map3k14 were increased whereas the level of Psmb10 was found to be reduced in TWEAK-Tg mice compared to littermate control mice (n = 3 in each group). B). QRT-PCR analysis showed that the levels of Notch1, Pgam2, Ankrd1, and TCap were reduced in TWEAK-Tg mice compared to control mice (n = 3 in each group). C). Western blot analysis of NF-κB2, Notch1 and TIMP2 protein expression profiles in TWEAK-Tg compared to control mice. The gel pictures presented here from two independent experiments (n = 4) showed that protein levels of Notch1, and TIMP-2 were significantly reduced whereas NF-κB2 protein levels were increased in gastrocnemius muscle of TWEAK-Tg compared to littermate control.