Fig. 8.

Ribosomal proteins RPL10A and RPL12 exist in lipid raft-enriched membranes and contain thioester bonds. A, 10 μg of proteins from the cytoplasmic, non-raft, and lipid raft-enriched fractions was separated by SDS-PAGE, electrotransferred, and immunoblotted with anti-RPL10A and anti-RPL12 antibodies. B, validation of the existence of thioester bonds in RPL10A and RPL12 proteins using ABE/immunoblotting. Proteins containing thioester bonds and controls were ABE-purified from equal amounts of lipid raft-enriched fractions in the presence and absence of HA, respectively. RPL10A and RPL12 proteins were detected by Western blotting. As a control, portions of the unpurified samples (i.e. total proteins) were also blotted. Data represent at least three independent experiments.