Figure 2.

Immunohistochemistry (IHC) detection of PrP^CWD using MAb F99/97.6.1 in various tissues of orally inoculated red deer (Cervus elaphus elaphus) with CWD.

1. Brain, medulla oblongata, obex, dorsal motor nucleus of vagus nucleus. There is moderate diffuse vacuolation of the neuropil and also occasional presence of vacuoles within neuronal perikarya. Hematoxylin & eosin. Bar = 30 μm.
2. Brain, medulla oblongata, obex. There is extensive accumulation of PrP^CWD (stained red) predominantly in the neuropil and with prominent perineuronal labelling. IHC. Bar = 70 μm.
3. Tonsil. Detection of PrP^CWD in tonsillar lymphoid follicles. IHC. Bar = 275 μm.
4. Colon, myenteric plexus. IHC. Bar = 70 μm.
5. Adrenal gland, medulla. IHC. Bar = 70 μm.
6. Heart, ventricular septum. IHC. Bar = 40 μm.