Table 1.
PrPCWD distribution and comparative subjective assessment of the signal intensities of immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) from different brain and spinal cord regions from 4 orally inoculated CWD-positive red deer (Cervus elaphus elaphus)
| Animala ID | Spinal cordb |
Brainstem |
Cerebellum |
Midbrain |
Thalamus |
Cerebrum |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IHCc | ELISAd | IHC | ELISA | IHC | ELISA | IHC | ELISA | IHC | ELISA | IHC | ELISA | |
| 101 | +++ | 3.226 | +++ | >3.5 | +++ | 3.016 | +++ | > 3.5 | +++ | > 3.5 | ++ | 1.983 |
| 103a | +++ | 2.570 | +++ | 2.749 | +++ | 2.543 | ++ | 2.739 | ++ | 2.866 | +++ | 2.614 |
| 105a | +++ | 3.165 | +++ | 3.042 | +++ | 2.331 | +++ | 3.014 | +++ | 2.995 | +++ | 3.010 |
| 106 | +++ | 3.227 | +++ | > 3.5 | ++ | 1.362 | +++ | 3.434 | +++ | > 3.5 | ++ | 1.736 |
a
Animals no. 103 and 105 developed clinical signs at 576 and 583 d post-inoculation, respectively. All deer were subsequently euthanized and subjected to postmortem examination and PrPCWD testing.
b
Sample of cervical spinal cord (C1–C4).
c
PrPCWD was detected by IHC with MAb F99/97.6.6 with the scoring system (+ to +++) described in Materials and methods.
d
Optical density (OD) data from Bio-Rad Laboratories’ ELISA.