Table 2.

Comparison of immunohistochemical (IHC) staining and enzyme-linked immunosorbent assay (ELISA) detection of PrP^CWD in selected tissues from 4 orally-inoculated CWD-positive red deer (Cervus elaphus elaphus)

	Detection of PrPCWD			Detection of PrPCWD
System, anatomical sites	ELISA	IHCa	System, anatomical sites	ELISA	IHC
Central nervous system			Pre-femoral LN	1/4	3/4
Eye	3/4b	4/4	Popliteal LN	4/4	3/4
Cervical spinal cord (C1–C3):	4/4	4/4	Spleen	3/4	3/4
Medulla (at obex)	4/4	4/4	Tonsil	3/4	4/4
Rostral medulla	4/4	4/4	Digestive system
Midbrain	4/4	4/4	Submandibular salivary gland	2/4	0/4
Thalamus	4/4	4/4	Parotid salivary gland	2/4	1/4
Frontal cortex	4/4	4/4	Reticulum	3/4	4/4
Cerebellum/vermis	4/4	4/4	Rumen	0/4	2/4
Peripheral nervous system			Abomasum	1/4	4/4
Trigeminal ganglia	1/4	4/4	Duodenum	2/3	3/3
Dorsal root ganglia	0/3c	3/3	Jejunum	0/4	4/4
Sympathetic trunk	0/4	0/4	Ileum	4/4	4/4
Vagus nerve	0/4	0/4	Proximal Colon	3/4	4/4
Sciatic nerve	0/4	0/4	Distal Colon	1/4	4/4
Radial nerve	0/4	0/4	Respiratory system
Facial nerve	0/4	0/4	Nasal mucosa	3/3	2/4
Brachial plexus	0/4	0/4	Lung	0/4	0/4
Lymphoreticular system			Endocrine system
Rectal lymphoid tissue	2/4	4/4	Pancreas	1/4	2/4
Peyer’s Patches	3/4	4/4	Thyroid Gland	0/4	1/4
Retropharyngeal LN	3/4	4/4	Adrenal Gland	3/3	3/3
Parotid LN	3/4	4/4	Pituitary Gland	4/4	4/4
Submandibular LN	3/4	4/4	Musculoskeletal system
Pre-Scapular LN	1/4	3/4	Diaphragm	0/4	0/4
Hepatic LN	3/4	4/4	Masseter muscle	0/4	0/4
Ruminal LN	3/3	3/4	Triceps brachii muscle	0/4	0/4
Abomasal LN	3/3	3/3	Longissimus thoracis muscle	0/4	0/4
Mesenteric LN	4/4	4/4	Semitendinosus muscle	0/4	0/4
Ileocecal LN	4/4	4/4
Sublumbar LN	2/3	3/3	Heart	2/4	4/4

^aPrP^CWD was detected by IHC with MAb F99/97.6.6.

^bResults are expressed as the number of test positive animals over the total number of animals examined.

^cSome tissues were not available for examination.

LN = lymph node.