Figure 5.

Determination of MHV-68-peptide-specific CD8⁺ T cell prevalence by ELISpot analysis. Dilutions of splenic mononuclear cells were incubated with feeders, pulsed with the MHV-68 peptides p56 or p79, on plates coated with a mAb to IFN-γ. The number of peptide-specific spots developed with a biotinylated rat anti-mouse IFN-γ after 40–44 hr of culture was determined by subtracting the values for control cultures without the relevant peptide. Mean ± SD reciprocal frequencies from 5–6 individual spleens per time point are shown. Reciprocal peptide-specific frequencies for naive I-A^b+/+ and I-A^b−/− mice were >100,000.