Table 2.
MHV-68-specific CD8+ T cell response in the BAL and spleen
| Mice | Time | Percentage IFN-γ positive of the total CD8+ population after a 6-hr incubation with peptide
|
|||
|---|---|---|---|---|---|
| BAL
|
Spleen
|
||||
| p56 | p79 | p56 | p79 | ||
| I-Ab+/+ | Day 14 | 24.2 | 23.3 | 2.2 ± 0.9 | 2.3 ± 1.4 |
| Day 22 | 10.8 | 22.4 | 1.2 ± 0.5 | 3.5 ± 0.8 | |
| Day 81 | 3.4 | 11.1 | 0.9 ± 0.2 | 2.7 ± 0.5 | |
| I-Ab−/− | Day 14 | 20.9 | 10.0 | 3.3 ± 1.1 | 3.4 ± 2.2 |
| Day 22 | 5.4 | 15.9 | 1.7 ± 0.7 | 2.6 ± 0.9 | |
| Day 81 | 5.6 | 4.9 | 4.2 ± 0.5 | 2.6 ± 0.8 | |
The spleen populations were enriched for the CD8+ set before staining as described in the legend to Table 1. All samples then were analyzed for response to the p56 and p79 MHV-68-peptides as described in the legend to Fig. 4. The spleen results are the mean ± SD for groups of five mice, while the BAL populations were analyzed as pools. At day 81, paired t tests showed a significantly greater response to p56 in the I-Ab−/− mice (P < 0.0001). These results were reproduced in four further experiments.