Figure 1.

Identification of alternatively spliced transcripts using second generation sequencing data. The 454 sequencing reads that span exon junctions provide information about alternatively spliced variants in the human transcriptome. For example, two typical alternatively spliced variants ('a' and 'b') of a single gene are shown in the top half of the figure. The lower half of the figure shows how different 454 reads may align to cDNA sequences generated from the mRNA providing evidence of alternatively splicing events.