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. 2009 Dec 30;10:643. doi: 10.1186/1471-2164-10-643

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Copyright ©2009 Grant-Downton et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MicroRNA induced cleavage of At3g19890; detection of cleavage products by modified 5' RACE. A. 5' RACE to identify miR774a-directed cleavage products. B. Watson-Crick base pairing and pattern of cleavage of At3g19890 by miR774a. C. Gel image showing products of 5' RACE reaction to detect ath-MIR2939-directed cleavage. D. Watson-Crick base pairing and pattern of cleavage of At3g19890 by ath-MIR2939. E. RT-PCR detection of mature ath-MIR2939 in mature pollen (MP) and leaf (L). For A and C, Lanes C1 and C2 are controls (minus 5' RACE adapter primer and minus gene specific primer). L, DNA ladder.