Table 3. B. rapa EST analysis identified putative orthologs to highly nectary-enriched Arabidopsis genes.
Arabidopsis locus | TAIR Annotation | AtMLN/Refa | AtMMN/Refa | No. of ESTsb |
At1g74820 | cupin family proteinc | 6.20 | 405.88 | 324 |
At1g65980 | peroxiredoxin type 2, putative | 4.34 | 0.28 | 220 |
At2g39060 | nodulin MtN3 family proteinc | 193.32 | 179.31 | 114 |
At2g36190 | beta-fructosidase, putatived | 66.63 | 62.00 | 59 |
At1g23010 | multi-copper oxidase type I family protein | 26.07 | 26.49 | 27 |
At3g27810 | myb family transcription factorc | 10.59 | 9.50 | 23 |
At1g69180 | transcription factor CRC (CRABS CLAW) | 198.09 | 251.69 | 22 |
At1g19640 | S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase (JMT)c | 33.04 | 7.24 | 15 |
At2g42830 | agamous-like MADS box protein AGL5 | 42.37 | 37.65 | 14 |
At4g12530 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein | 376.33 | 352.76 | 14 |
At1g77110 | PIN6 (PIN-FORMED 6); auxin:hydrogen symporter/transporterd | 84.81 | 90.87 | 8 |
At1g51340 | MATE efflux family proteinc | 37.65 | 32.61 | 1 |
At1g23300 | MATE efflux family proteinc | 171.12 | 58.88 | 1 |
Fold difference in normalized microarray probe signal intensity for Arabidopsis MLN and MMN over the average of seventeen individual reference tissues (described in [17]). All genes listed here displayed a minimum two-fold increase in mean probe set signal intensity in MLN and/or MMN over each individual reference tissue, along with t-test p-values and false discovery rate (FDR) q-values of <0.05. Full lists of genes meeting these cutoff criteria via Arabidopsis microarray, along with average probe intensities and p and q-values for individual nectary samples, and corresponding number of B. rapa EST hits, are available in Tables S10 (MLN) and S11 (MMN). A total of 207 putative B. rapa orthologs to Arabidopsis nectary-enriched genes (MLN and/or MMN) were identified through this analysis.
Number of B. rapa ESTs from Table S9 (ESTs from all libraries combined) generating hits against Arabidopsis locus.
Nectary-enriched expression of ortholog in B. rapa verified by RT PCR, results shown in Fig. 3.
Expression patterns also previously confirmed for orthologs to At2g36190 [16] and At1g77110 (Ruhlmann and Carter, in preparation).