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. 2010 Jan 20;5(1):e8801. doi: 10.1371/journal.pone.0008801

Figure 3. Measurement of small molecule uptake.

Figure 3

Hoescht 33342 is a substrate of the major AcrAB-TolC efflux systems. Accumulation by E. coli MG1655 and EAEC 042 was measured fluorometrically in the presence or absence of the efflux pump inhibitor PAβN and the proton-motive force inhibitor CCCP. Efflux of Hoescht 33342 is inhibited in the presence of both PAβN and CCCP in EAEC 042 and E. coli MG1655. However, Hoescht 33342 accumulates to higher levels in EAEC 042 than E. coli MG1655 suggesting EAEC 042 possesses a more permeable membrane.