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. 2009 Nov 5;29(1):14–26. doi: 10.1038/emboj.2009.316

Figure 4.

Figure 4

Antagonistic regulation of actin-based motility by actin-related protein (Arp)2/3 complex and tropomyosin. (A): low neural Wiskott–Aldrich syndrome protein (N-WASP) surface density (d=6 nm); (B): high N-WASP surface density (d=3 nm). Upper left panels: bead velocity varies in opposite directions with Arp2/3 concentration in the absence (empty circles) or in the presence of skeletal muscle tropomyosin (skTm) (filled circles). Upper right panels: histograms of the ratio of the velocity measured in the absence and in the presence of skTm. Bottom panels: phase contrast images of N-WASP functionalised beads and their actin tails in the presence of skTm (1.03 μM) and Arp2/3 complex as indicated. Scale bar 20 μm. Conditions: 7 μM F-actin, 2.6 μM profilin, 6.1 μM (A) or 5.2 μM (B) actin-depolymerising factor (ADF), 175 nM gelsolin. Results expressed as mean±s.d., n=13–32 beads from 2–5 different fields in each condition.