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. 2009 Nov 5;29(1):196–208. doi: 10.1038/emboj.2009.318

Figure 6.

Figure 6

Social isolation stress increases marble-burying behaviour and induces 5-HT receptor 5B (Htr5b) mRNA expression. (A) Marble-burying behaviour of group- and isolation-reared WT mice was examined (n=10–12 of C57BL/6 congenic mice for each group). (B) Real-time RT–PCR analysis of Htr5b mRNA was performed using RNAs from the brainstem of group- and isolation-reared WT mice (n=3). (C) Htr5b mRNA expression in the dorsal raphe nuclei of group- and isolation-reared WT mice was examined by in situ hybridization, as described in Figure 2D. (D) The group-reared WT and Atf-7−/− mice, and the isolation-reared Atf-7−/− mice were used for marble-burying tests (n=10–12 of C57BL/6 congenic mice for each group). (E) Real-time RT–PCR analysis of Htr5b mRNA was performed using RNAs from the brainstems of the mice described in D (n=3). **P<0.01, ***P<0.001.