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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Hum Mol Genet. 2003 Sep 23;12(22):2941–2948. doi: 10.1093/hmg/ddg319

Figure 1.

Figure 1

Mutation of the GT6 motif in the HS3 core element. A diagram of the human β-globin locus is shown in the upper panel. The five DNase I-hypersensitive sites (HS1–HS5) that comprise the LCR are indicated by arrows upstream of the five β-like globin genes, which are represented as dark boxes. 3′HS1 is shown by the arrow located downstream of the β-globin gene. The core element resides on a 225 bp HphI–Fnu4HI fragment. The wild-type sequence is shown with the GT motifs (GGTGG) in bold letters; each GT site is labeled above the sequence (GT1–GT7). GATA-1 sites are underlined and the single NF-E2 motif just upstream of the HphI restriction site is shown in italics. The base substitutions used to mutate the GT6 motif (boxed) are shown underneath the wild-type sequence. The mutation results in the introduction of a BamHI restriction site. Genbank humhbb coordinates (accession number U01317) are shown on the left.