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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Hum Mol Genet. 2003 Sep 23;12(22):2941–2948. doi: 10.1093/hmg/ddg319

Figure 3.

Figure 3

Structural analysis of GT6m β-YAC transgenic lines. A major problem using YACs as trangenes to produce transgenic mice is the high frequency of deletions within the transferred YACs. We utilize pulsed-field gel electrophoresis followed by Southern blot hybridization analysis to determine the structural integrity of the integrated β-YAC transgene. (A) The figure shows the 140 kb SfiI fragment encompassing most of the β-globin locus from 5′HS3 to the breakpoint of HPFH6 353 kb downstream of the β-globin gene. This various probes used are indicated on the diagram of the β-YAC and in Materials and Methods. (B) Autoradiograms of transgenic lines 1–3 carrying the GT6m β-YAC. Each line contains a single 140 kb SfiI fragment signifying an intact β-globin locus is integrated into the mouse genome. The first lane in each autoradiogram contains DNA from MEL cell line containing a single intact β-YAC, as determined previously by structural analysis and fluorescent in situ hybridization.