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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Hum Mol Genet. 2003 Sep 23;12(22):2941–2948. doi: 10.1093/hmg/ddg319

Figure 4.

Figure 4

Southern blot analyses to detect HS4 and PCR analyses to con-firm the presence of the GT6m mutation in the GT6m β-YAC transgenic lines. (A) Southern blot hybridization analysis showing that HS4 resides on a 10.4 kb EcoRI fragment along with HS3 and HS2. Each line produces a fragment that migrates at the same position as the wild-type control. The 9.5 kb fragment is of the murine thy1.1 gene used for copy number analysis. (B) PCR analysis followed by gel electrophoresis to detect BamHI site created by mutating the GT6 motif. As seen in the ethidium bromide stained agarose gel, PCR ampli-fication followed by digestion with BamHI results in a single 456 fragment in the wild-type lane (BamHI resistant) and two fragments of 308 and 148 bp in the GT6m transgenic lines.