Skip to main content
. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Blood. 2004 Dec 30;105(8):3346–3352. doi: 10.1182/blood-2004-03-0987

Figure 1. Transgene constructs.

Figure 1

All transgene constructs contain a transcription unit that comprises a truncated β-globin promoter (βpr; □) and γ-globin coding segment (γ gene) containing a partial deletion (▽) of the second intron (■, exons; black line, untranslated regions). Construct HS-40/βpr/γ gene contains the HS-40 element from the human α-globin locus (αHS-40; Inline graphic). Construct HS-40(mut)/βpr/γ gene contains the HS-40 element with a G-to-T transversion in the 3′ NF-E2 (nuclear factor-erythroid 2) motif. Construct μLCR/βpr/γ gene contains a 2.5-kb composite μLCR (▩). Construct HS-40/βpr/γ gene/hHS5 contains HS-40 and a 3.2-kb fragment encompassing the hHS5 chromatin insulator (▨). Construct cHS4/HS-40/βpr/γ gene contains HS-40 and a 1.2-kb fragment encompassing the cHS4 chromatin insulator (▧).