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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Blood. 2004 Dec 30;105(8):3346–3352. doi: 10.1182/blood-2004-03-0987

Figure 2. Analyses of structure and expression of construct HS-40/βpr/γ gene in transgenic mice.

Figure 2

Four lines (A–D) carrying this transgene were established. (Top) Structure analysis. DNA from 2 individual animals of each line was digested with NcoI, which cuts once within the construct, and analyzed by Southern blotting with the use of a probe for HS-40. The arrow to the left indicates the 1.7-kb repetitive fragments generated from head-to-tail repeats of the construct. Other bands were from the end fragments containing the HS-40 fragment of the construct and various sequences from the mouse genome. (Bottom) RNA analysis. Total RNA was prepared from the blood of 3- to 4-week-old mice, and RNase protection assay was performed with the use of RNA probes for human γ-globin (Hu γ) and mouse α-globin mRNAs (Mu α). The leftmost lane contains control RNA from cells expressing human γ-globin mRNA at 1.5% of mouse 3-globin mRNA.