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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Biochim Biophys Acta. 2005 Feb 14;1727(2):125–133. doi: 10.1016/j.bbaexp.2004.12.007

Fig. 6.

Fig. 6

Role of CCAAT motifs for the activity of GATA-1 on the KLF13 gene promoter. They were disrupted by point mutations listed in Table 1. Promoters lacking the distal, proximal and both CCAAT motifs are referred to CCAATmut1, CCAATmut2 and CCAATmut3, respectively, which are shown in the left panel. The numbers shown above are the distance to the transcription start site in kb. The right panel shows the relative luciferase activities tested in K562 cells. Luciferase counts were corrected by the β-Gal activity, and the average count of wild type (WT) promoter without co-expressed GATA-1 was considered as 100%. Error bars indicate one S.D. Solid lines shown at the right indicate that the promoter activities were significantly different. Results were obtained from three independent transfections in triplicate.